Cyst morphology and sequence analysis of the small subunit rDNA and ef1α identifies a novel Giardia genotype in a quenda
نویسندگان
چکیده
Sequence analysis of the small subunit ribosomal DNA (SSU-rDNA) and elongation factor 1 alpha (ef1α) was performed on Giardia cysts isolated from faeces collected from a quenda (Isoodon obesulus) in the southwest of Western Australia. The SSU-rDNA and ef1α were sequenced in their entirety and correspondingly aligned with the published sequence information of other known species and genotypes of Giardia. Phylogenetic analysis of the SSU-rDNA and ef1α sequences identified the quenda isolate as a novel genotype of Giardia not previously reported. We believe that this quenda Giardia isolate constitutes a distinct species, which may be endemic within the Australian native fauna. Keywords; Giardia; Small subunit rDNA; Ef1α; Phylogenetic; Marsupial
منابع مشابه
Morphology and Phylogeny of Scrippsiella trochoidea (Dinophyceae) a potentially harmful bloom forming species isolated from the sediments of Iran’s south coast
Phytoplankton cells and resting cysts of the species Scrippsiella trochoidea are regular and dominant components of the dinoflagellate flora of coastal marine waters and sediments around the world. This species is a common harmful bloom forming species in coastal waters. In this study, for the first time cyst of S. trochoidea were isolated from the sediments of southeast coast of Iran. Five str...
متن کاملGiardia in Western Australian wildlife.
Giardia has been found in numerous species of mammalian wildlife but very little information is available on the species and strains/genotypes that occur naturally in mammals in the wild. Recently, a novel genotype of Giardia was described in Western Australia, in the Southern brown bandicoot, or quenda (Isoodon obesulus). In order to determine the host range, distribution and prevalence of thi...
متن کاملAssessment of Chattonella subsalsa Cyst and Excysted Cells Isolateted from Sediment of Lipar Zone (Makran Coast) Based on Morphology and LSU-rDNAGen Sequence
This study has been carried out in order to identify the phytoplankton cysts from sediment of Lipar zone (southeast coast of Iran) based on morphology and phylogeny of germinated cell. The sediment samples were collected from three sations by Ekman grab with 225 cm2 collecting area in 2015. Unknown cysts with different morphology were cultured in the petridishei containing the F2 medium and kep...
متن کاملMolecular identification of Tilletia controversa and T. caries, the causal agent of wheat dwarf and common bunt
Common and dwarf bunt of wheat are recognized as being caused by three closely related species, Tilletia caries and T. laevis as common bunt, and T. controversa as dwarf bunt. The morphological characteristics of two species including T. controversa and T. caries were studied from wheat grown in two provinces of Iran, Lorestan and Chaharmahal–o-Bakhtiari duri...
متن کاملMorphology and Phylogeny of Scrippsiella trochoidea (Dinophyceae) a potentially harmful bloom forming species isolated from the sediments of Iran’s south coast
Phytoplankton cells and resting cysts of the species Scrippsiella trochoidea are regular and dominant components of the dinoflagellate flora of coastal marine waters and sediments around the world. This species is a common harmful bloom forming species in coastal waters. In this study, for the first time cyst of S. trochoidea were isolated from the sediments of southeast coast of Iran...
متن کامل